Nov 30, 2023
BIOL 2010 - Cell and Tissue Biology for Biofabrication Credits: 3
Hours/Week: Lecture 3 Lab None
Course Description: This course provides students earning the biofabrication certificate a foundation in cell and tissue biology required to succeed in the field of biofabrication. The course covers cell and tissue types, including those used in bioprinting, components of the extracellular matrix, factors that influence cell and tissue maturation, bioink components, sterile technique, staining, imaging, and microscopy, biohazardous materials and safety, and steps involved in 3D printing biological material. Culturing cells and tissues requires adherence to aseptic or sterile technique. The course includes a variety of hands-on projects applying knowledge of cells and tissues to the field of biofabrication.
Prerequisite(s): BIOL 1010 or BIOL 1020 or BIOL 1041 with a grade C or higher
- Review of cell components, including the importance of the extracellular matrix
- Basic tissue structure and different types of tissues
- Factors that influence cell and tissue maturation
- Cell and tissue culture methods
- Physiological limits of diffusion as it relates to bioprinting
- Process of bioink preparation, different types of bioinks, crosslinking techniques, including review of polymers
- Microscopy and imaging techniques, including anatomical planes and staining for cell viability
- Sterile technique in a variety of settings
- Biohazards of using living tissues
At the end of this course, students will be able to:
- classify the various cell types used in tissue culture and 3D bioprinting, including embryonic, induced embryonic, and pluripotent stem cells.
- distinguish between various tissue types, including the challenges to bioprinting each type
- analyze the advantages and disadvantages of various bioinks used in bioprinting to determine appropriate bioink for a particular 3D construct.
- compare and perform advanced forms of staining, imaging, and microscopy used in cell and tissue culture for 3D bioprinting.
- explain factors that influence cell and tissue maturation.
- demonstrate proper handling of biohazardous material.
- demonstrate proper and consistent use of sterile technique
- apply content knowledge and skills to print a project made of biological material.
- analyze printed project for viability and maturation using appropriate stains and microscopy.
Competency 1 (1-6)
Competency 2 (7-10)
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