BIOL 2010 - Cell and Tissue Biology for Biofabrication

1. Review of cell components, including the importance of the extracellular matrix
2. Basic tissue structure and different types of tissues
3. Factors that influence cell and tissue maturation
4. Cell and tissue culture methods
5. Physiological limits of diffusion as it relates to bioprinting
6. Process of bioink preparation, different types of bioinks, crosslinking techniques, including review of polymers
7. Microscopy and imaging techniques, including anatomical planes and staining for cell viability
8. Sterile technique in a variety of settings
9. Biohazards of using living tissues

1. classify the various cell types used in tissue culture and 3D bioprinting, including embryonic, induced embryonic, and pluripotent stem cells. 
2. distinguish between various tissue types, including the challenges to bioprinting each type
3. analyze the advantages and disadvantages of various bioinks used in bioprinting to determine appropriate bioink for a particular 3D construct.
4. compare and perform advanced forms of staining, imaging, and microscopy used in cell and tissue culture for 3D bioprinting.
5. explain factors that influence cell and tissue maturation.
6. demonstrate proper handling of biohazardous material.
7. demonstrate proper and consistent use of sterile technique
8. apply content knowledge and skills to print a project made of biological material.
9. analyze printed project for viability and maturation using appropriate stains and microscopy.

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